Abbelight SAFe Systems

Single-Molecule Localization Microscopy

Modular Nanoscopy Solutions

The SAFe nanoscopes can be incorporated onto any Olympus inverted microscope that features a cameraport, making it an easy and flexible nanoscopy solution.

Our IX85 microscope is well suited for nanoscopy applications due to its stable frame, TruFocus™ Z-drift compensation module, and open structure.

SAFe nanoscopes can also be combined with our FV4000 confocal laser scanning microscope and the IXplore™SpinSR super-resolution microscope system, enabling researchers to maximize their imaging capabilities with confocal microscopy, TIRFM, and SMLM in one system.

A Complete SMLM Solution

Abbelight provides a complete solution enabling even SMLM novices to achieve successful results during their first experiment.

Preparation:

A good experiment starts with good sample preparation. Abbelight offers ready-to-use and optimized SMLM kits for dSTORM and photo-activated localization microscopy (PALM).

Imaging:

Each system can be customized and upgraded to meet user needs and is run on Abbelight’s intuitive and easy-to-use software.

Analysis:

Abbelight’s NEO software offers a complete analysis workflow helping users to translate their data into meaningful results.

Support:

Throughout the experimental workflow, a dedicated Abbelight expert is assigned to support each user. Users can also acquire expertise through the online Abbelight Academy, which offers guides, video tutorials, and best practices.

Wide FOV TIRF Imaging with Homogenous Illumination

Olympus is a pioneer in the TIRF microscopy field, and our range of TIRF objectives is designed to provide tight control over the evanescent wave produced in TIRF imaging with magnifications ranging from 60X to 150X. The APON100XHOTIRF objective has the world’s highest NA of 1.7*, while the UPLAPO60XOHR and UPLAPO100XOHR are the world’s first plan apochromat objectives with a NA of 1.5.*

With Olympus’ optics and Abbelight’s ASTER illumination technology, users can achieve homogenous TIRF illumination over a wide FOV.

Cultured hippocampal neurons stained for spectrin cytoskeleton and imaged in TIRF microscopy mode. Homogeneous TIRF over the entire field of view of a Hamamatsu Fusion sCMOS camera (larger than the camera port size) was achieved using Abbelight ASTER technology. Sample courtesy of C. Leterrier, NeuroCytoLab, Marseille, and images by Adrien Mau, ISMO, Orsay.

U2OS cells stained for microtubules (alpha-Tubulin antibody) CF660, mitochondria (anti-TOMM20) CF680, and chromatin (EdU) AF647. Simultaneous multicolor 2D dSTORM with spectral demixing.

Spectral Demixing: Multicolor Imaging with One Laser, One Buffer, and One Acquisition

Although 3D nanoscopy has revolutionized the fluorescence microscopy field by attaining unprecedented resolutions, multicolor imaging remains challenging in SMLM. This difficulty is due to several factors, including chromatic aberrations, the choice of buffers, and the choice of single-molecule-compatible dyes.

To solve this challenge, Abbelight has implemented spectral demixing for SMLM. By separating far-red dyes using a dichroic cube and ratiometric algorithms, spectral demixing elegantly enables simultaneous multicolor imaging in SMLM.

Powerful Software for SMLM Data Analysis

Unlike standard fluorescence microscopy, which generates pixel-based images, SMLM produces point clouds with millions of localizations and associated uncertainties. Abbelight’s NEO software translates this data into a user-friendly package, simplifying data acquisition, and providing real-time image reconstruction and quantitative feedback.

NEO software also offers powerful tools to process SMLM data to study spatial and temporal distribution of localized single molecules. This includes cluster analysis using DBSCAN, Voronoi, and colocalization algorithms as CBC or single-particle tracking algorithms.

SALES

Peter Lindelöf (Lund)

E-mail: Peter@lri.se

Phone: 0708 - 13 35 94

Joakim Lindelöf (Lund)

E-mail: Joakim@lri.se

Phone: 0708 - 13 35 92

Kennet Agnebo (Stockholm)

E-mail: Kennet@lri.se

Phone: 0761 - 88 14 48

Hugo Lindelöf (Uppsala)

E-mail: Hugo@lri.se